Study of Binding Kinetics and Specificity of ^99mTc-SSS-Complex and ^99mTc-HMPAO to Blood Cells

Figure 1

iTLC (a) and HPLC (b) of the radiolabelled compound. Measuring the area under the curve of each peak, the labelling efficiency of ^99mTc-SSS-complex is 98.2% as calculated by iTLC and 95.8% as calculated by HPLC. The two peaks (free ^99mTc and ^99mTc-SSS) at radiochromatogram in b are wider than the UV peaks because the volume of the UV cell is only 10 µl and the volume of radiochromatogram cell is 50 µl for sensitivity reasons.

(a)

(b)