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Contrast Media & Molecular Imaging
Volume 2018 (2018), Article ID 6268437, 11 pages
Research Article

Neural Induction Potential and MRI of ADSCs Labeled Cationic Superparamagnetic Iron Oxide Nanoparticle In Vitro

1Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, The Second Affiliated Hospital of South China University of Technology, Guangzhou, Guangdong 511457, China
2Department of Radiology, Huizhou Municipal Central Hospital, Huizhou, Guangdong 516001, China
3School of Materials Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China
4Karmanos Cancer Institute, Wayne State University, 3990 John R. Street, Detroit, MI 48201, USA

Correspondence should be addressed to Qi Xie and Baolin Zhang

Received 24 August 2017; Revised 21 December 2017; Accepted 31 December 2017; Published 14 February 2018

Academic Editor: Maria P. Morales

Copyright © 2018 Weiqiong Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Magnetic resonance imaging (MRI) combined with contrast agents is believed to be useful for stem cell tracking in vivo, and the aim of this research was to investigate the biosafety and neural induction of SD rat-originated adipose derived stem cells (ADSCs) using cationic superparamagnetic iron oxide (SPIO) nanoparticle which was synthesized by the improved polyol method, in order to allow visualization using in vitro MRI. The scan protocols were performed with T2-mapping sequence; meanwhile, the ultrastructure of labeled cells was observed by transmission electron microscopy (TEM) while the iron content was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES). After neural induction, nestin and NSE (neural markers) were obviously expressed. In vitro MRI showed that the cationic PEG/PEI-modified SPIO nanoparticles could achieve great relaxation performance and favourable longevity. And the ICP-AES quantified the lowest iron content that could be detected by MRI as 1.56~1.8 pg/cell. This study showed that the cationic SPIO could be directly used to label ADSCs, which could then inductively differentiate into nerve and be imaged by in vitro MRI, which would exhibit important guiding significance for the further in vivo MRI towards animal models with neurodegenerative disorders.