111In-DANBIRT In Vivo Molecular Imaging of Inflammatory Cells in Atherosclerosis
3-hour 111In-DANBIRT SPECT/CT imaging. Representative SPECT/CT image illustrates image analysis with the localization of the volume of the determined ROI in different imaging planes (axial, sagittal, and coronal), to correlate the SPECT date matched in this case to the descending aorta (red arrows depict the descending aorta) (a). Representative SPECT/CT 3D reconstruction shows low specific uptake and background ROIs in normal-diet-fed mice ((b); the image partially shows the determined ROI for the aortic arch in red color) compared to a HFD-fed mice (c) correlating with the increased atherosclerotic plaque development and identification of LFA-1+ cells inside of the plaque lesion by histologic analysis shown in Figure 3. Increased uptake of 111In-DANBIRT in the descending aorta (d) and aortic arch (e) of HFD-fed mice at baseline, 4 weeks, and 8 weeks. Repeated measures ANOVA with multiple comparisons was used for statistical analysis (;) (n of 4 per group).
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