Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [¹⁸F]Fluoro-PEG-Folate

Figure 4

Representative immunohistochemical (HE) images of ED1⁺ and ED2⁺ macrophages in spleen sections of healthy () (ED1 and ED2), saline-treated (), and MTX-treated () rats. ((a), (b)) Images represent ED1⁺ macrophages in the spleen of saline-treated and MTX-treated rats, respectively. ((c), (d)) Isotype control stained spleen sections of saline-treated and MTX-treated rats, respectively. ((e), (f)) Images of ED2⁺ macrophages in the spleen of saline-treated and MTX-treated rats, respectively. ((g), (h)) Images of isotype control stained spleen sections of saline-treated and MTX-treated rats, respectively. ((I), (J)) Bar graph representations of quantifications of ED1⁺ and ED2⁺ macrophages in spleen of healthy, saline-treated, and MTX-treated rats. Values depict mean numbers of macrophages counted in predefined areas of the spleen. Error bars indicate SD. .