Iso-Osmolar Iodixanol Induces Less Increase in Circulating Endothelial Microparticles In Vivo and Less Endothelial Apoptosis In Vitro Compared with Low-Osmolar Iohexol

Figure 2

The effects of iodixanol and iohexol on viability and apoptosis of HUVECs. (a) HUVECs were stimulated with iodixanol or iohexol at different concentrations and time points and then cell proliferation/cytotoxicity assay was performed using CCK-8 assay. (b) HUVECs were treated with iodixanol or iohexol at different concentrations for 4 h and cell apoptosis was determined by Annexin V and PI staining. (c) HUVECs were treated with 20 vol% of iodixanol or iohexol for 4 h and stained with Hoechst. Reference bar size in 20 μm. Magnification × 400 folds. The data were expressed as mean ± SD (). Complete growth medium served as control. , , and versus control; , , and versus iodixanol.

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