Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes

Figure 4

Assessment of released-DOX penetration derived from the implemented fluid dynamics model. (a–e) Fluorescence images measured at 3 min 10 s (a), 3 min 40 s (b), 4 min 10 s (c), 5 min 20 s (d), and 13 min 10 s (e). (f–j) Corresponding released-DOX penetration shown by the displacement field. For an easier visualization, only displacement vectors associated to voxels with sufficient fluorescence signal (i.e., greater than 5% of the maximum fluorescence signal intensity) are displayed. The principal component analysis allowed determining the main direction of released-DOX penetration, with 94% of relative displacement along the eigenvector 1 (k). The temporal profile of relative displacements along the eigenvector 1 equilibrated 7 minutes after the fluorescence onset (l).