Preclinical Imaging Biomarkers for Postischaemic Neurovascular Remodelling

Figure 6

In vivo multiphoton luminescence imaging using PEG-GNPs of cerebral vasculature in murine stroke models. (a) Experimental timeline, prior to which an open-skull cranial window was generated in a live mouse. Photothrombosis was induced within the vessels to generate the mouse model. (b) Multiphoton luminescence images of neurovasculature before and after induction of photothrombotic stroke with 5 and 40 nm sized PEG-GNPs. Scale bar, 100 µm. (c) Relative luminescence of extravascular tissues following photothrombosis. , one-tailed t-test, n = 3; values are mean with standard deviation. PEG-GNP, polyethylene glycosylated gold nanoparticle (republished with permission from the Royal Society of Chemistry [46]; permission conveyed through Copyright Clearance Center, Inc).