Research Article
Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission Tomography/Fluorescent Tracer [68Ga]Ga-TC3-OGDOTA
Figure 2
PET kinetics of [68Ga]Ga-TC3-OGDOTA retention in MCAO and sham-operated mouse brains. (a) Scheme of ROIs for PET signal integration over the left (occluded) and right (nonoccluded) brain halves. (b–i) PET signal kinetics and their analyses in MCAO and sham-operated brains. (b) Sample PET kinetics in MCAO brain halves showing linear approximation during the 2nd hour of signal acquisition. (c) The same for sham-operated brains. (d, e, h) Comparison of linear approximation slope (d) and graphic analysis Patlak constants (e) for MCAO brains. (h) Representative coronal and axial sections of Ki mapping in an MCAO mouse brain. Ki map was aligned with a CT mouse image manually as described in Methods. (f, g, i) The same as (d, e, h) but for sham-operated brains. Data were collected from 5 MCAO and 3 sham-operated mice.