Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission Tomography/Fluorescent Tracer [⁶⁸Ga]Ga-TC3-OGDOTA

Figure 3

PET kinetics of [⁶⁸Ga]Ga-TC3-OGDOTA retention in 5xFAD mouse brains and wild-type controls. (a) Scheme of ROIs for PET signal integration over the forebrain (fb) and hindbrain (hb, control area). (b–h) Relative (fb/hb) PET signal kinetics and their analyses in 5xFAD mouse brains compared to wild-type controls. (b) Relative PET kinetics in 5xFAD and control 8-month-old male brains were linearly approximated starting from ∼10 min timepoint. (c) Comparison of Patlak K_i for (b) kinetics. (d) Representative coronal and axial sections of K_i mapping in a wild-type (WT, left) and a 5xFAD (right) mouse brains. The K_i map was aligned with a CT mouse image manually as described in Methods. (e, g) Relative PET kinetics in 5xFAD and control male (e) and female (g) brains were linearly approximated starting from ∼10 min timepoint. (f) Comparison of Patlak K_i for (e) kinetics. (h) Comparison of Patlak K_i for (g) kinetics.