^99mTc-⁶⁸Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits

Table 6

Pros and cons of nanocolloid with different labelling method in the pre- and intraoperative setting.

Nanocolloid label and detection	Preoperative imaging		Operative detection
	Pros	Cons	Pros	Cons
^99mTc planar	(i) Well-assessed methodology	(i) Shine through phenomenon; (ii) Gross anatomic landmark	(i) 1 or 2 days protocol possible; (ii) 6-hour half-life enables ex vivo quantification of excised SLN; (iii) No tracer spread during surgery; (iv) Portable cameras available	(i) Difficulty to assess the depth of the signal
^99mTc SPET-CT	(i) Good anatomical localization; (ii) Shine through phenomenon less relevant	(i) Absolute quantification less accurate than with PET
^68Ga PET-CT	(i) Precise anatomical localization of the target; (ii) Better differentiation between the first and second echelon lymph nodes; (iii) Accurate quantification of the uptake (SUV)	(ii) Logistics	(i) Electronic collimation needed for gamma detection; (ii) New positron detectors assuring high sensitivity for superficial targets	(i) Thorough logistic organization; (ii) Radioprotection problems; (iii) Difficult depth assessment with gamma ray detectors
ICG		(i) Poor quality detection strictly depth-dependent	(i) High contrast and sensitivity, low noise background; (ii) Long-term signal availability up to many days; (iii) Easy use	(i) Obesity; (ii) Autofluorescence; (iii) The amount of fluorophore within tissue cannot be determined accurately by fluorescence intensity measurements