Multiplexed ¹²⁹Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells

Figure 1

GV gene constructs, monoclonal KPL-4 cells expressing the GV gene sets, and cell proliferation assay of the cells. (a) Schematics of the inserts of Tol2 vectors for transfection of humanized GV genes gvpA, gvpC16, gvpC20, and gvpC28. CMV_min: CMV minimal promoter. (b) Monoclonal KPL-4 cells stably expressing GV gene combinations in the presence of 1 μg/ml Dox, shown after pelleting. From top to bottom, monoclonal cells stably expressing gvpA and gvpC20 (GV_AC20), gvpA and gvpC28 (GV_AC28), gvpA, gvpC16, and gvpC20 (GV_AC16C20). (c) Effect of GV gene expression on cell proliferation of each monoclonal cell, probed using a WST-8 reagent at 72 hours after addition of Dox (Dox+) or an equivalent amount of PBS (Dox-) into the culture medium. Normalized absorbances of the average of 8 separate cultures of each monoclonal cell are shown. An asterisk () indicates statistical significance: , t-test. Error bars indicate SEM.

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