Multiplexed ¹²⁹Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells

Figure 2

Expression of GVLPs in human cells. (a) Schematic illustration of the protocol developed to purify GVLPs from mammalian cells. (b) TEM images of purified GVLPs from the cells: GV_AC20 (left), GV_AC28 (middle), and GV_AC16C20 (right). Scale bars indicate 100 nm (left), 500 nm (middle), and 100 nm (right). (c) Confocal microscopy images of GV_AC28 cells. Fluorescence images of EGFP (left), mKate2 (middle), and merged image of the two (right). Scale bars indicate 5 μm in all three images.

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