Long Noncoding RNA CASC2 Facilitated Wound Healing through miRNA-155/HIF-1α in Diabetic Foot Ulcers

Figure 6

Inhibition of HIF-1α overturned the effect of miR-155 downregulation on fibroblasts. (a) The relative miR-155’s expression and HIF-1α were detected by RT-qPCR. (b, c) Flow cytometry to evaluate the apoptosis of fibroblasts. (d) The proliferation activity of the cell was measured by MTT. (e) Healing of wound assay to determine the cell's ability of migration at 0 h and 24 h. (f) The gel and histogram of HIF-1α, VEGF, and Col-I proteins in fibroblasts were detected by Western blot. and against group of DFU, , and against a group of DFU + si-miR-155.

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