Exogenous H₂S Protects against Septic Cardiomyopathy by Inhibiting Autophagy through the AMPK/mTOR Pathway

Figure 2

Exogenous H2S inhibits excessive autophagy to reduce myocardial injury. (a) The expression of autophagy-related protein by Western blot analysis. (b) Representative images of myocardial autophagosome ultrastructural morphology underwent different treatments. Scale bar. = 5 μm, 1 μm (c) Representative TUNEL staining images; Scale bar = 50 μm ((d)–(j)). The quantitative analysis of p-AMPK, p-mtor, beclin-1, p62, LC3-II, cleaved-caspase-3 and cleaved-caspase-9 by image J. (k) The number of autophagosomes in different treatment groups. All data are expressed as the mean ± SD. (l) Percentage of TUNEL-positive nuclei. vs. sham group; vs. CLP group. All the experiments were repeated three times.

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