[Retracted] Helicid Improves Lipopolysaccharide-Induced Apoptosis of C6 Cells by Regulating SH2D5 DNA Methylation via the CytC/Caspase9/Caspase3 Signaling Pathway

Figure 7

The MethPrimer online software predicts the CpG island in SH2D5 promoter region and the influence of helicid and 5-AzaD on SH2D5 expression. (a) It is predicted that the promoter region of SH2D5 gene contains CpG island. (b) BSP primers targeting CpG island in SH2D5 promoter region were designed by methyl primer software, and CpG dinucleotide was highlighted. (c) The expression of SH2D5 was detected by qRT-PCR. (d) Protein bands. (e) Statistical diagram of SH2D5 protein expression. <0.001, control vs LPS; ^##<0.01; ^###<0.001, LPS vs LPS + HEL; ^andand<0.01; ^andandand<0.001, LPS vs LPS+5-AzaD. All data are expressed as the mean ± SEM.

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