Quantitative Model for Efficient Temporal Targeting of Tumor Cells and Neovasculature
Figure 1
Confocal micrographs of tissue cross sections harvested from tumor-bearing mice at 24 h after injection with imaging-nanocells. Mice were injected with nanocells labeled with semiconductor nanocrystals (quantum dots) to monitor the distribution and leakage from the vessels in the tumor and normal tissues. The sections were immunostained for von Willebrand Factor (vWF) to delineate vasculature. Images were captured using a Zeiss LSM510 confocal microscope at pixel resolution. The sections were excited with a 488 nm laser, and emission was absorbed at FITC (vWF) and Rhodamine (QD) wavelengths. The nanocells were found to be spatially restricted within normal vasculature as seen in the overlap (yellow) of the red and green signal in the merge images but extravasate out from the tumor vasculature at 24 h as seen by the predominantly red signal (merge).
