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Computational and Mathematical Methods in Medicine
Volume 2013, Article ID 381356, 11 pages
http://dx.doi.org/10.1155/2013/381356
Research Article

Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons

1Department of Physiology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan
2Graduate School of Science and Engineering, Saitama University, Saitama, Saitama 338-8570, Japan
3Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan

Received 12 January 2013; Revised 18 March 2013; Accepted 18 April 2013

Academic Editor: Younghae Do

Copyright © 2013 Yoshihiro Ujihara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber, “Geometric control of cell life and death,” Science, vol. 276, no. 5317, pp. 1425–1428, 1997. View at Publisher · View at Google Scholar · View at Scopus
  2. D. E. Ingber, “Fibronectin controls capillary endothelial cell growth by modulating cell shape,” Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 9, pp. 3579–3583, 1990. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Singhvi, A. Kumar, G. P. Lopez et al., “Engineering cell shape and function,” Science, vol. 264, no. 5159, pp. 696–698, 1994. View at Google Scholar · View at Scopus
  4. V. M. Weaver, S. Lelièvre, J. N. Lakins et al., “β4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium,” Cancer Cell, vol. 2, no. 3, pp. 205–216, 2002. View at Publisher · View at Google Scholar · View at Scopus
  5. S. R. Heidemann, S. Kaech, R. E. Buxbaum, and A. Matus, “Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts,” Journal of Cell Biology, vol. 145, no. 1, pp. 109–122, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. T. Lecuit and P. F. Lenne, “Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis,” Nature Reviews Molecular Cell Biology, vol. 8, no. 8, pp. 633–644, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. G. T. Charras and M. A. Horton, “Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation,” Biophysical Journal, vol. 82, no. 6, pp. 2970–2981, 2002. View at Google Scholar · View at Scopus
  8. D. E. Ingber, D. Prusty, Z. Sun, H. Betensky, and N. Wang, “Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis,” Journal of Biomechanics, vol. 28, no. 12, pp. 1471–1484, 1995. View at Publisher · View at Google Scholar · View at Scopus
  9. I. Karl and J. Bereiter-Hahn, “Cell contraction caused by microtubule disruption is accompanied by shape changes and an increased elasticity measured by scanning acoustic microscopy,” Cell Biochemistry and Biophysics, vol. 29, no. 3, pp. 225–241, 1998. View at Google Scholar · View at Scopus
  10. A. Kim and W. Matthew Petroll, “Microtubule regulation of corneal fibroblast morphology and mechanical activity in 3-D culture,” Experimental Eye Research, vol. 85, no. 4, pp. 546–556, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. K. Nagayama and T. Matsumoto, “Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate,” American Journal of Physiology, vol. 295, no. 6, pp. C1569–C1578, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. Y. Ujihara, H. Miyazaki, and S. Wada, “Morphological study of fibroblasts treated with cytochalasin D and colchicine using a confocal laser scanning microscopy,” The Journal of Physiological Sciences, vol. 58, no. 7, pp. 499–506, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. R. Adams and L. Bischof, “Seeded region growing,” IEEE Transactions on Pattern Analysis and Machine Intelligence, vol. 16, no. 6, pp. 641–647, 1994. View at Google Scholar
  14. P. Bamford and B. Lovell, “Unsupervised cell nucleus segmentation with active contours,” Signal Processing, vol. 71, no. 2, pp. 203–213, 1998. View at Google Scholar · View at Scopus
  15. D. Cremers, M. Rousson, and R. Deriche, “A review of statistical approaches to level set segmentation: integrating color, texture, motion and shape,” International Journal of Computer Vision, vol. 72, no. 2, pp. 195–215, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. S. L. Horowitz and T. Pavlidis, “Picture segmentation by tree traversal algorithm,” Journal of the Association for Computing Machinery, vol. 23, no. 2, pp. 368–388, 1976. View at Google Scholar · View at Scopus
  17. M. Kass, A. Witkin, and D. Terzopoulos, “Snakes: active contour models,” International Journal of Computer Vision, vol. 1, no. 4, pp. 321–331, 1988. View at Publisher · View at Google Scholar · View at Scopus
  18. E. Sifakis, C. Garcia, and G. Tziritas, “Bayesian level sets for image segmentation,” Journal of Visual Communication and Image Representation, vol. 13, no. 1-2, pp. 44–64, 2002. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Yezzi, A. Tsai, and A. Willsky, “A fully global approach to image segmentation via coupled curve evolution equations,” Journal of Visual Communication and Image Representation, vol. 13, no. 1-2, pp. 195–216, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. C. Wählby, J. Lindblad, M. Vondrus, E. Bengtsson, and L. Björkesten, “Algorithms for cytoplasm segmentation of fluorescence labelled cells,” Analytical Cellular Pathology, vol. 24, no. 2-3, pp. 101–111, 2002. View at Google Scholar · View at Scopus
  21. R. C. Gonzalez and R. E. Woods, Digital Image Processing, Prentice Hall, New Jersey, NJ, USA, 2nd edition, 2002.
  22. M. Nakamura, S. Wada, T. Miki, Y. Shimada, Y. Suda, and G. Tamura, “Automated segmentation and morphometric analysis of the human airway tree from multidetector CT images,” The Journal of Physiological Sciences, vol. 58, no. 7, pp. 493–498, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. D. E. Ingber, “Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton,” Journal of Cell Science, vol. 104, no. 3, pp. 613–627, 1993. View at Google Scholar · View at Scopus
  24. D. Choquet, D. P. Felsenfeld, and M. P. Sheetz, “Extracellular matrix rigidity causes strengthening of integrin- cytoskeleton linkages,” Cell, vol. 88, no. 1, pp. 39–48, 1997. View at Google Scholar · View at Scopus
  25. D. E. Ingber, “Tensegrity II. How structural networks influence cellular information processing networks,” Journal of Cell Science, vol. 116, no. 8, pp. 1397–1408, 2003. View at Publisher · View at Google Scholar · View at Scopus