Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons

Table 1

The summary of the volume, adhesion area, and fibroblast height of the three cell groups.

	CON (𝑛=42)	CD (𝑛=50)	COL (𝑛=46)
Volume 𝑉 (μm^3)
Proposed method	2742 ± 1529	2165 ± 1151	2201 ± 949
Conventional method	2440 ± 1340*	1777 ± 917∗#	1811 ± 743∗#
Adhesion area 𝑆𝑎 (μm^2)
Proposed method	989 ± 480	357 ± 178^$	722 ± 389^#
Conventional method	902 ± 454*	320 ± 157∗$	609 ± 327∗#
Height 𝐻 (μm)
Proposed method	7.4 ± 2.0	10.7 ± 1.6^$	7.5 ± 2.4
Conventional method	7.5 ± 2.0*	10.7 ± 1.7∗$	7.5 ± 2.4*

∗P < 0.05 versus proposed method (paired Student’s t-test).
^#P < 0.05 versus CON, ^$P < 0.05 versus CON and COL (Bonferroni correction).