A New Multichannel Spectral Imaging Laser Scanning Confocal Microscope

Figure 1

Diagram of the confocal scanning head. A: Laser combination and selection module, B: confocal scanning imaging module, C: multi-channel spectral imaging module, 1: dichroic mirror for spare laser combination, 2–5: dichroic mirror for four lasers combination, 6: mirror, 7: acousto-optical tunable filter (AOTF), 8: dichroic mirror for excitation and fluorescence separation, 9-10: X-Y galvanometer optical scanner, 11: scanning lens, 12: tube lens, 13: objective lens, 14: sample slide, 15: pinhole lens, 16: pinhole, 17: collimation lens, 18: prism, 19: focal lens, 20: movable slit 1, 21, 24, 27: PMTs, 22, 25: relay lens, 23, 26: movable slit 2, 3.