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Computational and Mathematical Methods in Medicine
Volume 2014, Article ID 454310, 6 pages
Research Article

Genomic and Functional Analysis of the Toxic Effect of Tachyplesin I on the Embryonic Development of Zebrafish

1Industrial Center, Shenzhen Polytechnic, Shenzhen 518055, China
2School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen 518055, China

Received 27 February 2014; Accepted 1 April 2014; Published 29 April 2014

Academic Editor: Tao Huang

Copyright © 2014 Hongya Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tachyplesin I (TP I) is an antimicrobial peptide isolated from the hemocytes of the horseshoe crab. With the developments of DNA microarray technology, the genetic analysis of the toxic effect of TP I on embryo was originally considered in our recent study. Based on our microarray data of the embryonic samples of zebrafish treated with the different doses of TP I, we performed a series of statistical data analyses to explore the toxic effect of TP I at the genomic level. In this paper, we first employed the hexaMplot to illustrate the continuous variation of the gene expressions of the embryonic cells treated with the different doses of TP I. The probabilistic model-based Hough transform was used to classify these differentially coexpressed genes of TP I on the zebrafish embryos. As a result, three line rays supported with the corresponding 174 genes were detected in our analysis. Some biological processes of the featured genes, such as antigen processing, nuclear chromatin, and structural constituent of eye lens, were significantly filtered with the smaller values.