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Computational and Mathematical Methods in Medicine
Volume 2015, Article ID 471076, 8 pages
http://dx.doi.org/10.1155/2015/471076
Research Article

Cell Pluripotency Levels Associated with Imprinted Genes in Human

1Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China
2BasePair BioTechonology Co., Ltd., Shanghai 200235, China
3Shanghai Center for Bioinformation Technology, Shanghai 200235, China

Received 20 January 2015; Revised 16 March 2015; Accepted 17 March 2015

Academic Editor: Tao Huang

Copyright © 2015 Liyun Yuan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pluripotent stem cells are exhibited similarly in the morphology, gene expression, growth properties, and epigenetic modification with embryonic stem cells (ESCs). However, it is still controversial that the pluripotency of induced pluripotent stem cell (iPSC) is much inferior to ESC, and the differentiation capacity of iPSC and ESC can also be separated by transcriptome and epigenetics. miRNAs, which act in posttranscriptional regulation of gene expression and are involved in many basic cellular processes, may reveal the answer. In this paper, we focused on identifying the hidden relationship between miRNAs and imprinted genes in cell pluripotency. Total miRNA expression patterns in iPSC and ES cells were comprehensively analysed and linked with human imprinted genes, which show a global picture of their potential function in pluripotent level. A new CPA4-KLF14 region which locates in chromosomal homologous segments (CHSs) within mammals and include both imprinted genes and significantly expressed miRNAs was first identified. Molecular network analysis showed genes interacted with imprinted genes closely and enriched in modules such as cancer, cell death and survival, and tumor morphology. This imprinted region may provide a new look for those who are interested in cell pluripotency of hiPSCs and hESCs.