Towards Automated Three-Dimensional Tracking of Nephrons through Stacked Histological Image Sets

Figure 7

A manually tracked mouse nephron is shown on the left. The same nephron is successfully tracked automatically by the algorithm (with %) and is shown on the right. Tracking terminates automatically at the glomerulus. Note that, in each plot, the cortex is shown at the bottom and the DTL extends upwards. The path is coloured by the error, or residual, with respect to the manually tracked nephron. Slight discrepancies in appearance are due to different image alignments and different point coordinates used by the two methods. The distal DTL has greater error simply because the manual path was not tracked as far (therefore, %). It can be seen in the error histogram that most of the residuals are less than 15 pixels. The correct paths of the PCT, PST, and DTL are tracked.