Modeling of the Bacillus subtilis Bacterial Biofilm Growing on an Agar Substrate

Figure 2

Characterization of growing Bacillus subtilis biofilm. (a) Time-lapse images of a growing Bacillus subtilis biofilm growing on top of nutritive agar medium at 12-hour intervals. Upon excitation, the motile, matrix-producing, and sporulating phenotypes produce different fluorescent colors and are observed using red, blue, and yellow filters, respectively. The transmission images are shown at the bottom and are used to determine the optical density. Green line serves as guide indicating at 48 hours that the extension of the matrix-producing region is comparable to the motile cells and exceeds that of the sporulating cells.

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