Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences

Figure 1

Consecutive time frames from 11 to 30 (b) show that a prominent fusion event corresponds to the patch of interest in a TIRFM image sequence (a). indicates the fusion pore opening, that is, the initiation of a fusion event. indicates the initiation of a diffusion process. Here, transition time is 1.6 s (8 frames, sampling rate is 5 frames/s).