Boolean Modeling of Cellular and Molecular Pathways Involved in Influenza Infection

Figure 5

Regulation of IL2 by NFAT inferred by dynamic Boolean model: (a) network of interactions between IL2 and the TFs regulating its expression assembled from literature, (b) enhanced regulatory network inferred using dynamic Boolean model, and (c) the expression of IL2 mRNA evaluated by RT-PCR in DCs treated for one hour with medium containing the NFAT inhibitors CsA (CsA), VIVIT peptide (VIVIT), or vehicle (−), and then left mock-infected (mock) or infected with IV during 2 hours. The expression levels of IL2 in infected cells were normalized to the levels of nontreated, mock-infected cells.

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