Improved Specificity and False Discovery Rates for Multiplex Analysis of Changes in Strain-Specific Anti-Influenza IgG

Figure 3

The examples of standard curve and 5PL fitting formula examples generated by mPlex-Flu assay. (a) The standard curves generated with anti-IgG capture antibodies commonly used in traditional immunoassays through fitting a five-parameter logistic regression model. (b–d) The representative subtype-specific standard curves of influenza HAs from total 45 strains, A/Indonesia/5/2005 (A/Ind05, H5), A/Japan/305/1957 (A/Jap57, H2), and A/Viet Nam/1203/2004 (A/Viet04, H5), generated using five-parameter logistic regression models with different parameters for different subtypes. For each graph, the blue markers and line represent a standard curve, either a single curve for multiple strains (a) or a single strain-specific curve (b–d).

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