[Retracted] Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Figure 4

ASF1B interacts and colocalizes with BCAR1 in LUAD. (a) Heat map showing the correlation between ASF1B and several BCAR family genes. (b) Scatter plot showing the correlation between ASF1B and BCAR1 in LUAD. (c) Binding between BCAR1 and ASF1B verified by immunoprecipitation. Cell lysates were immunoprecipitated with the anti-BCAR1 antibody. The input served as the positive control and IgG as the negative control. (d) Colocalization of ASF1B with BCAR1. Immunofluorescent signals for DAPI (blue) distributed in the nuclei. Green signals stand for ASF1B, and red signals represent BCAR1. The yellow signals indicate colocalization. (e) Western blot analysis of BCAR1 protein expression after ASF1B overexpression. (f) Western blot analysis of ASF1B protein expression after BCAR1 overexpression.

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