Angelica sinensis Polysaccharide Alleviates Myocardial Fibrosis and Oxidative Stress in the Heart of Hypertensive Rats

Figure 2

ASP treatment inhibits cardiac fibrosis in vivo. H&E (a) and Masson’s (b) staining assays were performed to demonstrate the effects of ASP on cardiac fibrosis of rat left ventricular tissues. (c) Immunohistochemical analysis of α-SMA and TGF-β1 protein levels in rat left ventricular tissues. (d) LVMI was measured in the different sample groups. The expression levels of collagen I, α-SMA, fibronectin, and vimentin were evaluated by performing RT-qPCR (e) and western blot assays (f) in the different sample groups. Protein expression levels relative to β-actin levels are shown. Three independent experiments were performed. The graph shows the calculated for at least three experiments. ^{or #}, ^{or ##}.

(a)

(b)

(c)

(d)

(e)

(f)