[Retracted] lncENST Suppress the Warburg Effect Regulating the Tumor Progress by the Nkx2-5/ErbB2 Axis in Hepatocellular Carcinoma

Figure 6

Effect of Nkx2-5 on ErbB2 expression in HCC-xenografted tumor tissues. (a) Immunofluorescence staining of ErbB2 and Nkx2-5 (punctate green dots in white rectangles) in tumor tissues of HCC-xenografted mice subjected to RFA and lnc-INT/OV injection. , ×100. Relative (b) mRNA and (c) protein expression of ErbB2 in tumor tissues of HCC-xenografted mice subjected to RFA and lnc-INT/OV injection. (d) Bioinformatic analysis based on the Champion Chip Transcription Factor Search Portal from Qiagen revealed the binding site between the transcription factor (TF in the image) Nkx2-5 and the ErbB2 gene. (e) Chromatin immunoprecipitation of the Nkx2-5 protein and the ErbB2 gene. Pull down was performed using an antibody against Nkx2-5. The expression of ErbB2 was detected in SMMC-7721 cells treated with lnc/Nkx-OV/INT. For all, the data are expressed as the , , .

(a)

(b)

(c)

(d)

(e)