[Retracted] ACE2 Promotes the Synthesis of Pulmonary Surfactant to Improve AT II Cell Injury via SIRT1/eNOS Pathway

Figure 2

ACE2 alleviated the hypoxia-induced inflammation and apoptosis in AT II cells. The transfection efficiency of was evaluated by qRT-PCR. (b) The ACE2 expression detected by western blot. (c) The expressions of AT II cell markers and PS were detected by western blot. (d) The effects of hypoxia and pcDNA3.1-ACE2 on cell viability were tested by MTT assay. (e) HMGB1 and inflammatory cytokines (p-NFκB, IL-6, MCP-1, and TGFβ1) were detected by western blot. (f) ELISA was used to detect the levels of Ang-II and Ang-(1-7) in AT II cell lysate mediated by ACE2. (g) Cell apoptosis was detected by flow cytometry. ; .

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