[Retracted] ACE2 Promotes the Synthesis of Pulmonary Surfactant to Improve AT II Cell Injury via SIRT1/eNOS Pathway

Figure 3

ACE2 attenuated AT II cell damage through the SIRT1/eNOS pathway. (a) the levels of SIRT1/eNOS-related proteins after the addition of EX527 were detected by western blot. (b) The effects of inhibitor EX527 and pcDNA3.1-ACE2 on the activation of SIRT1/eNOS were measured by western blot. (c) The detection of CK-8, KGF, and PS expressions by western blot was carried out. (d) MTT assay was used to test cell viability. (e) The expressions of HMGB1 and inflammatory cytokines in AT II cells after different treatments were measured by western blot. (f) The levels of Ang-II and Ang-(1-7) in AT II cells lysate after different treatments were tested by ELISA. (g) Flow cytometry was used for cell apoptosis analysis. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)