[Retracted] Circular RNA circFAT1(e2) Promotes Colorectal Cancer Tumorigenesis via the miR-30e-5p/ITGA6 Axis

Figure 4

miR-30e-5p was a direct binding site of circFAT1(e2). (a, b) The dual-luciferase report assay in 293T cells transfected with luciferase report plasmids containing circFAT1(e2) (circFAT1(e2)3UTR-WT or circFAT1(e2)3UTR-mut) with mimic-NC, miR-30e-5p, or miR-30e-5p-in. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC. miR-30e-5p-in vs. inhibit-NC. miR-30e-5p-in represents the miR-30e-5p inhibitor. (c) The qRT-PCR assays of the relative expression level of miR-30e-5p to mimic-NC in CRC cells transfected with si-circFAT1(e2) or si-NC. Error bars represent the of at least three independent experiments. vs. the control group. (d) The qRT-PCR assays of relative circFAT1(e2) expression to mimic-NC in CRC cells transfected with mimic-NC or miR-30e-5p. Error bars represent the of at least three independent experiments. (e) The miR-30e-5p expression levels as determined by qRT-PCR in CRC cells transfected with mimic-NC, miR-30e-5p, or miR-30e-5p-in. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC. miR-30e-5p-in vs. inhibit-NC. miR-30e-5p-in represents the miR-30e-5p inhibitor. (f) G CCK-8 assay in CRC cells transfected with mimic-NC, miR-30e-5p, or miR-30e-5p-in. Error bars represent the of at least three independent experiments. and miR-30e-5p vs. mimic-NC. and miR-30e-5p-in vs. inhibit-NC. miR-30e-5p-in represents the miR-30e-5p inhibitor. (h, i) Transwell migration assay in HCT116 cells transfected with miR-30e-5p or mimic-NC by Lipofectamine 2000. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC. (j, k) Transwell invasion assay in RKO cells transfected with miR-30e-5p or mimic-NC by Lipofectamine 2000. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC.

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