[Retracted] Circular RNA circFAT1(e2) Promotes Colorectal Cancer Tumorigenesis via the miR-30e-5p/ITGA6 Axis

Figure 5

ITGA6 acted as a potential downstream binding site of miR-30e-5p in the process of CRC. (a, b) Dual-luciferase assay in 293T cells transfected with luciferase report plasmids containing ITGA6 3UTR (wild type or mutant) with mimic-NC, miR-30e-5p, or miR-30e-5p-in. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC. miR-30e-5p-in vs. inhibit-NC. (c) Relative mRNA of ITGA6 in 293T cells which transfected with miR-30e-5p-in, miR-30e-5p, or mimic-NC. Error bars represent the of at least three independent experiments. miR-30e-5p vs. mimic-NC. miR-30e-5p-in vs. inhibit-NC. (d) The expression levels of ITGA6 in 293T cells which transfected with si-ITGA6, miR-30e-5p-in, or mimic-NC. Error bars represent the of at least three independent experiments. si-ITGA6 vs. si-NC. miR-30e-5p-in vs. inhibit-NC. (e) Cell migration assay and (f) CCK-8 assay in 293T cells which transfected with si-ITGA6, miR-30e-5p-in, or mimic-NC. Error bars represent the of at least three independent experiments. vs. the control group.

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