Research Article

Bioinformatics Analysis of ceRNA Network Related to Polycystic Ovarian Syndrome

Figure 1

The reconstruction of the lncRNA-miRNA-mRNA network. Firstly, expression data of miRNA, lncRNA, and mRNA were downloaded from the Gene Expression Omnibus. Secondly, DEMs (differentially expressed mRNAs), DELs (differentially expressed lncRNAs), and DEMis (differentially expressed miRNAs) were screened at and . Thirdly, target lncRNAs of DEMis were predicted using RNAhybrid and miRanda, and target mRNAs of DEMis were predicted using miRTarBase and miRWalk. Fourthly, the coexpression lncRNAs and coexpression mRNAs were obtained to merge the target lncRNAs and mRNAs of DEMis with DELs and DEMs, respectively. Finally, the coexpression lncRNAs, DEMis, and coexpression mRNAs were mapped into the interactions.