High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma

Table 1

The reagent.

Reagent	Specifications	Serial number	Manufacturer
DMEM/F12 culture medium	BR	A4192001	Gibco
FBS	BR	10099133	Gibco
Trypsin	BR	1213981	Gibco
Hank’s buffer	500 ml	PB180321	Procell
TRIzol reagent	BR	DP424	TIANGEN
Reverse transcription kit		DBI-2220	DBI
Fluorescent quantitative PCR detection kit		AOPR-1200	Genecopies
DEPC		V900882	Vetec
Absolute ethanol, isopropanol, chloroform	AR		China
Metformin hydrochloride	0.5 g	h20023370	Sino
MTT cell proliferation test kit	500 t	MT-06	DOJINDO
Cyclin D antibody	10 μl	Ab273608	Abcam