High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma

Table 4

PCR reaction system.

2× all-in-one qPCR mix	10.0 μl
PCR forward primer (2 μM)	2.0 μl
PCR reverse primer (2 μM)	2.0 μl
cDNA	2.0 μl
50× Rox Reference Dye	0.4 μl
ddH2O	3.6 μl