High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma

Table 5

PCR amplification.

Cycles	Steps	Temperature	Time	Detection
1	Initial denaturation	95°C	10 min	No
40	Denaturation	95°C	10 s	No
	Annealing	55°C	20 s	No
	Extension	72°C	35 s	Yes