High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma

Table 6

Separating gel formula.

Separation gel concentration	8%	10%	12%	15%	18%	20%
H2O mL	4.63	4	3.3	2.3	1.3	0.63
30% acrylamide (29 : 1) mL	2.67	3.3	4	5	6	6.67
1.5 M Tris-HCl (pH 8.8) mL			2.5 mL
10%SDS mL			0.1 mL
AP mL			0.1 mL
TEMED μL			5 μL
Total volume mL			10 mL