High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma

Table 7

Concentrated gel formula.

Reagent	5% concentrated glue
H2O mL	2	3	4	6
30% acrylamide (29 : 1) mL	0.5	0.75	1	1.5
1 M Tris-HCl (pH 6.8) mL	0.5	0.75	1	1.5
10%SDS μL	40	60	80	120
AP μL	30	45	60	90
TEMED μL	4	6	8	12
Total volume mL	3	4.5	6	9