[Retracted] miR-335-5p Inhibits Progression of Uterine Leiomyoma by Targeting ARGLU1

Figure 2

Upregulation of miR-335-5p inhibits viability and proliferation, promotes apoptosis, and induces cell cycle arrest at G1 phase in UL cell lines. (a) The viability of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic was assessed by CCK-8 assay at indicated times. The proliferation of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic was assessed by (b) colony formation and (c) EdU assays. (d) The expression levels of proliferation-related proteins, including PCNA and Ki-67, were evaluated by western blot assay. (e) The cell cycle distribution of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic was assessed by flow cytometry analysis assay. (f) The expression levels of cell cycle-related proteins, including Cyclin A1, Cyclin B1, Cyclin D2, and p27, were evaluated by western blot assay. (g) The cell apoptosis of HCC1688 and SK-UT-1 cells transfected with miR-335-5p mimic was assessed by flow cytometry analysis assay. (h) The expression levels of cell apoptosis-related proteins, including Bcl-2, Bax, Cleaved caspase-3, and Cleaved caspase-9, were evaluated by western blot assay. vs. the NC mimic group. All data were presented as the . .

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