[Retracted] Development and Characterization of a Nanobody against Human T-Cell Immunoglobulin and Mucin-3

Figure 1

Phage display nanobody library construction. (a) Evaluation of immunization effect by ELISA. Wells were coated with either 1 μg/mL TIM-3 recombination protein or PBS. Serum before the first immunization and after the last immunization were detected by HRP-conjugated goat against Llama antibody and ABTS reagent. (b) The first PCR production had evident bands on 1000 bp and 750 bp. Lanes 1-4 represent different cDNA template quantities. (c) The 400 bp VHH fragments were amplified in second PCR. (d) 20 colonies were randomly picked and detected by PCR to calculate ratio of insert (18 positive colonies in 20.90%). , compared to preimmunization.

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