[Retracted] Development and Characterization of a Nanobody against Human T-Cell Immunoglobulin and Mucin-3

Figure 4

SDS-PAGE and Western blots to confirm purity and size of TIM-3 nanobodies (about 15 kDa). (a) 4%-20% SDS-PAGE shows nanobodies purified by immobilized metal affinity chromatography (IMAC). (b) Nanobodies were detected by anti-HIS antibody (1 : 1000) and HRP-conjugated anti-mouse antibody (1 : 2000).

(a)

(b)