[Retracted] Long Noncoding RNA Zinc Finger Antisense 1 Affects Glucocorticoid-Induced Osteonecrosis of the Femoral Head by Performing as a ceRNA for MicroRNA-124-3p and Accelerating Transforming Growth Factor Type III Receptor

Figure 8

GCs impact osteoblast viability and bone formation by modulating the LncZFAS1/miR-124-3p/TGFBR3 axis. (a) RT-qPCR or Western blot to detect miR-124-3p and TGFBR3 in MC3T3-E1 cells after cotransfection of oe-LncZFAS1 and si-TGFBR3; (b) CCK-8 to detect the effect of the cotransfection of oe-LncZFAS1 and si-TGFBR3 on the viability of MC3T3-E1 cells; (c) flow cytometry to detect the effect of cotransfection of oe-LncZFAS1 and si-TGFBR3 on the apoptosis rate of MC3T3-E1 cells; (d) Alizarin red staining to detect the effect of cotransfection of oe-LncZFAS1 and si-TGFBR3 on the calcification ability of MC3T3-E1 cells; (e) ALP staining to detect the effect of cotransfection of oe-LncZFAS1 and si-TGFBR3 on ALP activity of MC3T3-E1 cells; (f) Western blot detection of the effect of cotransfection of oe-LncZFAS1 and si-TGFBR3 on BMP-9, BMP-3, and OCN in MC3T3-E1 cells.

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