miR-641 Inhibited Cell Proliferation and Induced Apoptosis by Targeting NUCKS1/PI3K/AKT Signaling Pathway in Breast Cancer

Figure 4

NUCKS1 was a direct target of miR-641 in breast cancer cells. The predicted complementary sequences for miR-641 in the 3UTR of NUCKS1 and the mutations; (b) miR-641 mimic or inhibitor significantly inhibited or promoted the luciferase activity that carried wt 3-UTR of NUCKS1 but had no obvious effect on mut 3-UTR of NUCKS1; (c) NUCKS1 mRNA expression was higher in the bio-miR-641-WT group than that in the bio-NC group after biotin-coupled miRNA sedimentation; (d) NUCKS1 mRNA and protein expression was measured in MDA-MB-231 and MCF7 cells transfected with mimic NC or miR-641 mimics. and vs. NC mimics or bio-NC.

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