[Retracted] GEO Database Screening Combined with In Vitro Experiments to Study the Mechanism of hsa_circ_0003570 in Infantile Hemangiomas

Figure 5

hsa_circ_0003570, as the ceRNA of hsa-miR-138-5p, upregulated the expression of RGS5. (a) Putative miRNA binding sites in hsa_circ_0003570 and RGS5. (b) Putative miRNA binding sites in RGS5. (c) Detection of luciferase activity after hsa-miR-138-5p mimic and pGL3-hsa_circ_0003570 WT or pGL3-hsa_circ_0003570 MUT. (d) Luciferase activity detection after transfection with hsa-miR-138-5p mimic and pGL3-RGS5 WT or pGL3-RGS5 MUT. (e) Relative expression level of hsa_circ_0003570, hsa-miR-138-5p, and RGS5 mRNA in HEMEC transfected with control, NC, si-hsa_circ_0003570, hsa-miR-138-5p mimic, si-hsa_circ_0003570+hsa-miR-138-5p inhibitor, and hsa-miR-138-5p inhibitor detected by qRT-PCR. (f) The relative expression of RGS5 in HEMEC after transfection with control, NC, si-hsa_circ_0003570, hsa-miR-138-5p mimic, si-hsa_circ_0003570+hsa-miR-138-5p inhibitor, and hsa-miR-138-5p inhibitor. , compared with control.

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