An Investigation into the Correlation of Intestinal Flora with Obesity and Gestational Diabetes Mellitus

Table 5

PCR amplification system.

Reagents	Dose
buffer	4 μl
2.5 mM dNTPs	2 μl
Forward primer (5 μM)	0.8 μl
Reverse primer (5 μM)	0.8 μl
FastPfu Polymerase	0.4 μl
BSA	0.2 μl
Template DNA	10 ng