MicroRNA-17-5p Protects against Propofol Anesthesia-Induced Neurotoxicity and Autophagy Impairment via Targeting BCL2L11

Figure 4

BCL2L11 is directly targeted by miR-17-5p in SH-SY5Y. (a) qRT-PCR examined the level of MIND, MYLIP, and BCL2L11 in SH-SY5Y cells with miR-17-5p or NC mimic transfection. (b, c) BCL2L11 protein levels were measured in SH-SY5Y cell posttransfection with miR-17-5p or NC mimics by WB. (d) Predicated binding region between miR-17-5p and BCL2L11 through Targetscan. (e) Validation of the combination between miR-17-5p and BCL2L11 via DLR assay. (f, g) BCL2L11 transcriptional and translational level was tested in SH-SY5Y cells treated with PPF or phosphate buffer by qRT-PCR and WB analysis, respectively. .

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