MicroRNA-17-5p Protects against Propofol Anesthesia-Induced Neurotoxicity and Autophagy Impairment via Targeting BCL2L11

Figure 5

The influence of miR-17-5p on SH-SY5Y viability and apoptosis via modulating BCL2L11. Postexposure with PPF (10 μg/ml) for 24 h, rescue experiments were conducted. (a, b) Overexpression efficacy of BCL2L11 in SH-SY5Y cells was, respectively, evaluated by qRT-PCR and WB evaluations. (c, d) MTT assay and FC analysis were performed for evaluating SH-SY5Y cell viability and apoptosis after transfection of NC mimics, miR-17-5p mimics, and . (e) The translational levels of Bcl-2, Bax, and Cleaved-caspase-3 were estimated by WB in SH-SY5Y cells with plasmids transfection. p, p, and p values less than 0.05, 0.01, and 0.001, accordingly.

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