Angiotensin II Mediates Cardiomyocyte Hypertrophy in Atrial Cardiomyopathy via Epigenetic Transcriptional Regulation

Figure 2

Identification the enrichment of MEF2, HDAC4, HDAC5, and histone modifications on the hypertrophy-related genes ANF and BNP in human atrial tissue from patients with atrial fibrillation (AF) or normal sinus rhythm (N). Quantitative ChIP analysis for MEF2, HDAC4, HDAC5, and H3K27ac and H4ac enrichment at the promoter of ANP (a) and BNP (b) from chromatin isolated from AF or N atrial tissue. Histone modifications H3K27ac and H4ac, which have been associated with activation of gene expression, were measured at ANF (a) and BNP (b) gene promoters by ChIP. per group. Data are shown as the , statistical significance was determined by one-way ANOVA followed by the Bonferroni posttest. vs. N group. All ChIP data are plotted as fold enrichment over equivalent amounts of input DNA, H3K27ac:H3 Lys27 acetylation, and H4ac:H4 Lys acetylation.

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