Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis

Figure 8

Chondrocytes were pretreated with 200 μg/mL of GBT for 1 h and then stimulated with 100 ng/mL LPS for 30 min or 12 h. (a) The expression of NFκB p65 in the nucleus and IκB-α in cells after 30 min of LPS stimulation and immunofluorescence of NFκB p65. (b) TLR4, MyD88, and TLR4-bound MyD88 after 30 min of LPS stimulation. (c) The expression of NLRP3, ASC, IL-18, and IL-1β after 12 h of LPS stimulation. (d) PI3K, P-PI3K (Tyr467), P-PI3K (Tyr199), AKT, and p-AKT (Ser473) after 12 h of LPS stimulation. (e) HIF-1α expression after 12 h of LPS stimulation. (f) TNF-α mRNA expression and TNF-α content in cell supernatant after 12 h of LPS stimulation. compared with blank-control group; ^# compared with LPS-induced group.

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